Cisplatin ic50 sh-sy5y

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WebAn IC50 curve for MCF7 cells treated with cisplatin. Percentage cell survival is plotted against the logarithm of treatment concentrations. Data points are the means±SD of duplicate... WebThe IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D3 through MTT assay in NRK-52E kidney cells were determined. DNA damage was signiﬁcantly increased (p < 0.05) compared to the control group in all groups except for vitamin D3.

Cytotoxic effect of gambogic acid on SH-SY5Y neuroblastoma ... - PubMed

WebSH-SY5Y were deposited to the ATCC(®) in 1970 by June L. Biedler.Three important characteristics of SH-SY5Y cells should be considered when using these cells in in vitro studies. First, cultures include both adherent and floating cells, both types of … WebScribd adalah situs bacaan dan penerbitan sosial terbesar di dunia. biocool - purify my drinking water 250 tbl

Knockdown of galectin-1 facilitated cisplatin sensitivity by …

WebJul 20, 2024 · In this analysis, 10 × 10 3 SH-SY5Y cells were plated in 96-wells. 10 mM stocks of ME0053, ME0055 and ME0192 compounds were diluted in DMEM to obtain the treatment doses of 50 µM, 100 µM, 150 µM and 200 µM. Treated- and control groups were incubated for 24 h at 37 °C and %5 CO 2. WebOct 30, 2012 · SH-SY5Y cells were seeded in 6-well plates (at 2×10 6 /ml) and incubated for 24 h until they were treated with 0.1 μM VCR for 6, 12, 18 and 24 h. Non-treated cells at 0 h were used as the control group. Cells were washed twice with ice-cold PBS and fixed with 70% ice-cold ethanol. WebJun 1, 2012 · Cisplatin (cis-diamminedichloroplatinum (II), cddp) is a DNA-damaging agent that forms platinum adducts in the DNA. This leads to intrastrand and interstrand … biocon training programme

Effect of CDDP and OHP treatment on SH-SY5Y cell

Cisplatin regulates SH-SY5Y cell growth through …

WebMay 11, 2016 · Table 3 Comparison of doxorubicin IC50 values of A549, 293T, HeLa, U2OS, and SH-SY5Y spheroids and effects over the incubation times Abbreviation: NR, IC50 not reached. After 24 hrs, 4 out of 5 of the spheroids did not show any significant change in their metabolic activity, when incubated with increasing concentrations of DXR. WebEnter the email address you signed up with and we'll email you a reset link. dahek traductionWebNov 26, 2024 · Cisplatin is one of the commonly used anticancer drugs in clinic chemotherapy, but drug resistance is a major therapeutic obstacle for successful treatment. ... recent studies indicated an effective inhibitory effect by ginkgolic acid C17:1, with IC50 values of less than 2 μM, ... in potassium chloride-induced α-synuclein aggregates in SH ... biocool clean water tank

"Webγ-Mangostin is a xanthone with hydroxyl groups that confer the substance-free radical scavenging effects. As opposed to the other more extensively studied mangostins, scarce research has been conducted on neuroprotective effects of γ-mangostin on " - Cisplatin ic50 sh-sy5y

Cisplatin ic50 sh-sy5y

An IC50 curve for MCF7 cells treated with cisplatin ... - ResearchGate

WebAs a result, compounds 2 and 5 exhibited potent neuroprotective activity against 6-OHDA-induced cell death in SH-SY5Y cells with the lowest IC50 value (0.5 μM) while compounds 1, 3 and 4 possessed significant neuroprotective activity with IC50 value less than 10 μM. WebOct 23, 2024 · Cisplatin 5 µg and different concentrations (Cisplatin + CoS NPs bioconjugates (5, 10, 25, 50, 75 μg) doses were applied to SH-SY5Y neuroblastoma cell lines for 24 h. TAC, TOS and MTT tests were performed 24 h after the application.

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WebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), … WebOct 1, 2024 · The purpose of this study was to evaluate the effect of 50-Hz electromagnetic field (EMF) in combination with CDDP and bleomycin (Bleo) on expression of some of DNA repair genes (GADD45A, XRCC1,...

WebSensitivity testing results, expressed as the attention of medicine called for to prevent by 50 % the development of infection in cell society (IC50), differ substantially relying on a … WebThe IC50 values of the two platinum derivatives varied considerably. The values for cisplatin varied between 0.022 microgram ml-1 and 0.56 microgram ml-1 and the corresponding values for carboplatin were 0.096-1.20 microgram ml-1. The range of the ratios between carboplatin IC50 and cisplatin IC50, from 1.5:1 to 4.4:1, was rather narrow.

WebJul 4, 2016 · Three-dimensional (3D) cell cultivation is a powerful technique for monitoring and understanding diverse cellular mechanisms in developmental cancer and neuronal biology, tissue engineering, and drug development. 3D systems could relate better to in vivo models than two-dimensional (2D) cultures. WebCisplatin 50 mg should be given once in 3 to 4 weeks. High dose of cisplatin 50 mg may cause severe neuropathy. Loss of motor nerve function may occur. Irregular liver …

WebJun 18, 2004 · Abstract In human SH-SY5Y neuroblastoma cells, two distinct intracellular Ca2+ stores, a KCl-/caffeine-sensitive and a carbachol-/IP3-sensitive store, were demonstrated previously. In this study, responses of these two intracellular Ca2+ stores to thapsigargin were characterized.

WebDrug: Cisplatin - Cancerrxgene - Genomics of Drug Sensitivity in Cancer Genomics of Drug Sensitivity in Cancer Compound: Cisplatin Drug Target: DNA crosslinker Drug Target … biocontrol science and tecWebcomplexes 2 and 3 resulting in greater cell uptake than cisplatin in selected tumor cell lines, only 3 showed comparable or higher antitumor activity. General low cytotoxicity of complex 2 in the tested cell lines (SH-SY5Y, SK-OV-3, Hep-G2, Caco-2, HeLa, MCF-7, MG-63, ZL-65) appeared to depend dahej pratha essay in englishWebIC50 values for (a-d) bortezomib-and (e-h) cisplatin-treated MCF7, HCC38, MCF-10A, and MDA-MB-436 breast cancer cells generated in the present study and pharmacoDB 42 datasets. No bar is shown... dahek full hd movieWebHere, we examined cellular cytotoxicity by cell viability assay and DNA fragmentation by DNA-ladder assay. Activation of different protein expressions were detected by western blot analyses. We first demonstrated that GA reduces the human SH-SY5Y neuroblastoma cell viability with IC50 of 1.28 μM at 6 h which has less toxicity in fibroblast cells. biocool safe waterWebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often … biocoop action charitativeWebOct 25, 2016 · The changing 50% inhibitory concentration (IC50) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer Oncotarget 2016 Oct 25;7 (43):70803-70821. doi: 10.18632/oncotarget.12223. Authors biocoop agde horairesWebThe SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH ( ATCC HTB-11 ), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research. Product category Human cells Organism biocool high concentrate