Peasy e1
WebJul 26, 2013 · The PCR products were gel-purified and cloned into pEASY -E1 expression vector (Transgen, China) according to the Usage Information of pEASY -E1 expression system. WebDownload pEASY-Blunt E2 (linearized).dna file. Download Plasmid Open in SnapGene. ... pEASY-Blunt E1 (linearized) pHY300PLK. pSMART HCAmp. T7 promoter. pEASY-Blunt E2 (linearized) pICOz. pSMART HCAmp (linearized) tac promoter. pEASY-Blunt Simple (linearized) pInvRecA. pSMART HCKan. trc promoter.
Peasy e1
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WebAug 1, 2024 · The recombinant plasmid pEasy-agkihpin was transformed into E. coli BL21 (DE3) and the recombinant cells were grown in LB culture medium (containing 50 μg/ml … WebPeasy Blunt E1 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …
WebApr 20, 2015 · The gene rebdh was subsequently over-expressed in Escherichia coli cells using the pEASY-E1 expression vector and the resulting recombinant ReBDH with the N-terminal His-tag was purified to homogeneity by nickel affinity chromatography. WebOct 18, 2024 · The expression vector pEASY-E1 (TransGen biotech, China) carrying a C-terminal His6-tag was used to clone and express catA. Cloning and sequencing of catA from R. ruber OA1: Rhodococcus ruber strain OA1 was cultured in LB broth at 37°C for 2 days and genomic DNA was extracted using the Bacterial gDNA Kit (BIOMIGA, China) for use as a …
WebFusion Expression Vector Peasy E1 Tatcp 1, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … WebJul 1, 2012 · After confirming the cloned fragments by DNA sequencing, pEasy-E1-CsTS was transformed into the Rosseta good (DE3) cells and the recombinant CsTS protein …
WebAfter IPTG induction, the pEASY-E1-FTL in E. coli BL21 (DE3) cells was separated using SDS-PAGE. A band of ~19 kDa was present in the precipitate of transformed cells (Fig. 3A, lane 4), which was...
WebJan 10, 2010 · The cell suspension of the transformant E. coli BL21 (DE3) pLysS/pEASY-E1/aiiA induced by IPTG was analyzed with SDS–PAGE. The apparent bands with molecular weight of about 28 kDa were detected on the gel in the cell lysates of E. coli BL21(DE3)/pEASY-E1/aiiA after incubation for 1–4 h induced with IPTG, but not in those … paula scott longendykeWebSamples analyzed included low molecular weight protein markers (M, lane 1), un-induced expression of pEASY-E1/ZmCAT in total BL21 (DE3) cell lysates (U, lane 2), 1 mM IPTG induced expression of ... paula scott penmansWebDec 17, 2024 · A pEASY-E1 plasmid that was N-terminally His6-tagged and had a T7 lac promoter was used for the precise regulation and efficient expression of the target genes. The pEASY-E1-AFE2726 plasmid expressing the AFE2726 gene was constructed as follows: the plasmid-pEASY-E1 was purchased from Beijing Quanshijin Biotechnology Company. paula scozzafava danbury ctWebAug 1, 2024 · The PCR fragment containing the r-agkihpin gene was purified and cloned into a pEasy-E1 vector to obtain the expression plasmid pEasy-agkihpin which included a His 6-tag (Fig. 1). The results of gene sequencing showed that the sequence of PCR product of pEasy-agkihpin was identical to the . Discussion. Agkihpin, ... paula’s choice clinical 1% retinol treatmentWebJan 8, 2016 · The LB medium with pEASY-EaCAT and pEASY-SoCAT was induced for 2 and 4 h at 37 °C, and 1.0 mL medium was collected at each time point. The LB medium with empty pEASY-E1 was induced with IPTG for 6 h, and 1.0 mL corresponding medium was collected. The medium collected was used for the SDS-PAGE with 25 μL loading sample. paula scott palmdaleWebOct 29, 2024 · The 2952-bp styS gene was amplified by PCR from the P. putida B3 genome using primers F/R-pEASY-styS ( Table 2 ), and the recombined plasmid pEASY-E1-styS was then constructed. Subsequently, the pEASY-E1-styS vector was introduced into E. coli BL21 (DE3), and the StyS protein was obtained by adopting the same method used for the StyR … paula scriva dance studioWebAfter IPTG induction, the pEASY-E1-FTL in E. coli BL21 (DE3) cells was separated using SDS-PAGE. A band of ~19 kDa was present in the precipitate of transformed cells (Fig. 3A, lane … paula scriva dance studio robinson pa